Microarray Analysis of BeWo and JEG3 Trophoblast Cell Lines: Identification of Differentially Expressed Transcripts

Burleigh, D.W.; Kendziorski, C.M.; Choi, Y.J.; Grindle, K.M.; Grendell, R.L.; Magness, R.R.; Golos, T.G.

doi:10.1016/j.placenta.2006.05.001

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Volume 28, Issue 5 , Pages 383-389, May 2007

Microarray Analysis of BeWo and JEG3 Trophoblast Cell Lines: Identification of Differentially Expressed Transcripts

D.W. Burleigh
- Wisconsin National Primate Research Center, University of Wisconsin Medical School, 1220 Capitol Court, Madison, WI 53715-1299, USA
- Department of Obstetrics and Gynecology, Perinatal Research Laboratories, University of Wisconsin Medical School, 1220 Capitol Court, Madison, WI 53715-1299, USA
- Current address: Richard Stockton College of New Jersey, Department of Biology, Pomona, NJ 08240, USA.
C.M. Kendziorski
- Department of Biostatistics and Medical Informatics, University of Wisconsin Medical School, 1220 Capitol Court, Madison, WI 53715-1299, USA
Y.J. Choi
- Department of Biostatistics and Medical Informatics, University of Wisconsin Medical School, 1220 Capitol Court, Madison, WI 53715-1299, USA
K.M. Grindle
- Department of Obstetrics and Gynecology, Perinatal Research Laboratories, University of Wisconsin Medical School, 1220 Capitol Court, Madison, WI 53715-1299, USA
R.L. Grendell
- Wisconsin National Primate Research Center, University of Wisconsin Medical School, 1220 Capitol Court, Madison, WI 53715-1299, USA
R.R. Magness
- Department of Obstetrics and Gynecology, Perinatal Research Laboratories, University of Wisconsin Medical School, 1220 Capitol Court, Madison, WI 53715-1299, USA
T.G. Golos
- Wisconsin National Primate Research Center, University of Wisconsin Medical School, 1220 Capitol Court, Madison, WI 53715-1299, USA
- Department of Obstetrics and Gynecology, Perinatal Research Laboratories, University of Wisconsin Medical School, 1220 Capitol Court, Madison, WI 53715-1299, USA
- Corresponding author. Tel.: +1 608 263 3567; fax: +1 608 263 3524.

Accepted 8 May 2006. published online 24 June 2006.

Abstract

Trophoblast cell lines are important research tools used as a surrogate for primary trophoblast cells in the study of placental function. Because the cellular origins of transformed trophoblasts are likely to be diverse, it would be of value to understand the unique and shared phenotypes of the cells on a global scale. We have compared two widely used cell lines, BeWo and JEG3, by microarray analysis in order to identify differentially expressed genes. Results indicated that approximately 2700 genes were differentially expressed between the cell lines, with principal differences observed in the biological processes of response to stress, cell adhesion, signal transduction, and protein and nucleobase metabolisms. These data suggest that BeWo and JEG3 cell lines, and perhaps other trophoblast cell lines, are sufficiently dissimilar from each other such that they will be differentially suited for specific experimental paradigms.

Keywords: BeWo, JEG3, Trophoblast cell lines, Microarray