Placenta
Volume 28, Issue 11 , Pages 1110-1117 , November 2007

Bovine Caruncular Epithelial Cell Line (BCEC-1) Isolated from the Placenta Forms a Functional Epithelial Barrier in a Polarised Cell Culture Model

  • P.S. Bridger

      Affiliations

    • Department of Veterinary Anatomy, Histology and Embryology, Justus-Liebig-University, Frankfurter Strasse 98, D-35392 Giessen, Germany
    • ,
  • C. Menge

      Affiliations

    • Institute for Hygiene and Infectious Diseases of Animals, Justus-Liebig-University, Frankfurter Strasse 85-89, D-35392 Giessen, Germany
    • ,
  • R. Leiser

      Affiliations

    • Department of Veterinary Anatomy, Histology and Embryology, Justus-Liebig-University, Frankfurter Strasse 98, D-35392 Giessen, Germany
    • ,
  • H.-R. Tinneberg

      Affiliations

    • Department of Obstetrics and Gynecology, Justus-Liebig-University, Klinikstrasse 32, D-35385 Giessen, Germany
    • ,
  • C.D. Pfarrer

      Affiliations

    • Department of Obstetrics and Gynecology, Justus-Liebig-University, Klinikstrasse 32, D-35385 Giessen, Germany
    • Corresponding Author InformationCorresponding author. Tel.: +49 641 99 45282; fax: +49 641 99 45258.

,Accepted 5 July 2007.

References 

  1. Wooding FB. Current topic: the synepitheliochorial placenta of ruminants: binucleate cell fusions and hormone production. Placenta. 1992;13:101–113
  2. Pfarrer CD, Hirsch P, Guillomot M, Leiser R. Interaction of integrin receptors with extracellular matrix is involved in trophoblast giant cell migration in bovine placentomes. Placenta. 2003;24:588–597
  3. Soundararajan R, Rao AJ. Trophoblast ‘pseudo-tumorigenesis’: significance and contributory factors. Reprod Biol Endocrinol. 2004;2:15
  4. Pfarrer CD, Heeb C, Leiser R. Expression of gap junctional connexins 26, 32 and 43 in bovine placentomes during pregnancy. Placenta. 2006;27:79–86
  5. Bücher K, Leiser R, Tiemann U, Pfarrer CD. Platelet-activating factor receptor (PAF-R) and acetylhydrolase (PAF-AH) are co-expressed in immature bovine trophoblast giant cells throughout gestation, but not at parturition. Prostaglandins Other Lipid Mediat. 2006;79:74–83
  6. Laven RA, Peters AR. Bovine retained placenta: aetiology, pathogenesis and economic loss. Vet Rec. 1996;139:465–471
  7. Fortier MA, Guilbault LA, Grasso F. Specific properties of epithelial and stromal cells from the endometrium of cows. J Reprod Fertil. 1988;83:239–248
  8. Horn S, Bathgate R, Lioutas C, Bracken K, Ivell R. Bovine endometrial epithelial cells as a model system to study oxytocin receptor regulation. Hum Reprod Update. 1998;4:605–614
  9. Banu SK, Arosh JA, Chapdelaine P, Fortier MA. Expression of prostaglandin transporter in the bovine uterus and fetal membranes during pregnancy. Biol Reprod. 2005;73:230–236
  10. Asselin E, Drolet P, Fortier MA. In vitro response to oxytocin and interferon-τ in bovine endometrial cells from caruncular and inter-caruncular areas. Biol Reprod. 1998;59:241–247
  11. Pfarrer CD, Ruziwa SD, Winther H, Callesen H, Leiser R, Schams D, et al. Localization of vascular endothelial growth factor (VEGF) and its receptors VEGFR-1 and VEGFR-2 in bovine placentomes from implantation until term. Placenta. 2006;27:889–898
  12. Shemesh M, Harel-Markowitz E, Gurevich M, Shore LS. Staurosporine stimulates progesterone production by bovine placental cells. Biol Reprod. 1994;51:146–151
  13. Johnson GA, Austin KJ, Collins AM, Murdoch WJ, Hansen TR. Endometrial ISG17 mRNA and a related mRNA are induced by interferon-tau and localized to glandular epithelial and stromal cells from pregnant cows. Endocrine. 1999;10:243–252
  14. Binelli M, Guzeloglu A, Badinga L, Arnold DR, Sirois J, Hansen TR, et al. Interferon-τ modulates phorbol ester-induced production of prostaglandin and expression of cyclooxygenase-2 and phospholipase-A2 from bovine endometrial cells. Biol Reprod. 2000;63:417–424
  15. Parent J, Fortier MA. Expression and contribution of three different isoforms of prostaglandin E synthase in the bovine endometrium. Biol Reprod. 2005;73:36–44
  16. Thiel HJ, Plagemann PGW, Moennig V. Pestiviruses. In:  Knipe DM,  Howley PM editor. Fields virology. Philadelphia: Lippincott-Raven; 1996;p. 1059–1073
  17. Bridger PS, Haupt S, Klisch K, Leiser R, Tinneberg HR, Pfarrer CD. Validation of epitheloid cell cultures isolated from the caruncle and the cotyledon of cattle throughout pregnancy. Theriogenology 2007; in press.
  18. Zeiler M, Leiser R, Johnson GA, Tinneberg HR, Pfarrer CD. Development of an in vitro model for bovine placentation: A comparison of the in vivo and in vitro expression of integrins and components of extracellular matrix in bovine placental cells. Cells Tissues Organs 2007; in press.
  19. Wille KH. Ueber die pränatale Entwicklung der Dickdarm-Mukosa unter besonderer Berücksichtigung des Epithels. Morphologische sowie histo- und zytochemische Untersuchungen am Blinddarm des Rindes. Habilitation Thesis, Justus-Liebig-University Giessen. Germany. 1984;3–4
  20. Corapi WV, Donis RO, Dubovi EJ. Monoclonal antibody analyses of cytopathic and noncytopathic viruses from fatal bovine viral diarrhea virus infections. J Virol. 1988;62:2823–2827
  21. Lackner T, ller A, Pankraz A, Becher P, Thiel HJ, Gorbalenya AE, et al. Temporal modulation of an autoprotease is crucial for replication and pathogenicity of an RNA virus. J Virol. 2004;78:10765–10775
  22. Corapi WV, Donis RO, Dubovi EJ. Characterization of a panel of monoclonal antibodies and their use in the study of the antigenic diversity of bovine viral diarrhea virus. Am J Vet Res. 1990;51:1388–1394
  23. Evans RM. Vimentin: The conundrum of the intermediate filament gene family. BioEssays. 1998;20:79–86
  24. Johnson GA, Burghardt RC, Bazer FW, Spencer TE, Newton GR. Development and characterization of immortalized ovine endometrial cell lines. Biol Reprod. 1999;61:1324–1330
  25. Galabova-Kovacs G, Walter I, Aurich C, Aurich JE. Steroid receptors in canine endometrial cells can be regulated by estrogen and progesterone under in vitro conditions. Theriogenology. 2004;61:963–976
  26. Classen-Linke I, Kusche M, Knauthe R, Beier HM. Establishment of a human endometrial cell culture system and characterization of its polarized hormone responsive epithelial cells. Cell Tissue Res. 1997;287:171–185
  27. Zhang Z, Paria BC, Davis DL. Pig endometrial cells in primary culture: morphology, secretion of prostaglandins and proteins, and effects of pregnancy. J Anim Sci. 1991;69:3005–3015
  28. Arslan A, Almazan G, Zingg HH. Characterization and co-culture of novel nontransformed cell lines derived from rat endometrial epithelium and stroma. In Vitro Cell Dev Biol Anim. 1995;31:140–148
  29. Munson L, Chandler SK, Schlafer DH. Cultivation of bovine fetal and adult endometrial epithelial cells. J Tissue Cult Methods. 1988;11:129–133
  30. Yamauchi N, Yamada O, Takahashi T, Imai K, Hashizume K, Sato T, et al. A three-dimensional cell culture model for bovine endometrium: Regeneration of a multicellular spheroid using ascorbate. Placenta. 2003;24:258–269
  31. Binelli M, Subramaniam P, Diaz T, Johnson GA, Hansen TR, Badinga L, et al. Bovine interferon-τ stimulates the Janus kinase-signal transducer and activator of transcription pathway in bovine endometrial epithelial cells. Biol Reprod. 2001;64:654–665
  32. Soule HD, Maloney TM, Wolman SR, Peterson WD, Brenz R, McGrath CM, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 1990;50:6075–6086
  33. Leiser R. Development of contact between trophoblast and uterine epithelium during the early stages of implantation in the cow. Zentralbl Veterinarmed [C]. 1975;4:63–86
  34. Pfarrer CD, Goebel S, Zeiler M, Leiser R, Tinneberg HR. Binding to a specific matrix enhances proliferation and expression of signalling molecules in bovine caruncular epithelial cells. Placenta. 2006;27
  35. Walia B, Castaneda FE, Wang L, Kolachala VL, Bajaj R, Roman J, et al. Polarized fibronectin secretion induced by adenosine regulates bacterial-epithelial interaction in human intestinal epithelial cells. Biochem J. 2004;382:589–596
  36. Hynes RO, Yamada KM. Fibronectins: Multifunctional modular glycoproteins. J Cell Biol. 1982;95:369–377
  37. Fredriksen B, Press CM, Loken T, Ïdegaard SA. Distribution of viral antigen in uterus, placenta and foetus of cattle persistently infected with bovine virus diarrhoea virus. Vet Microbiol. 1999;64:109–122
  38. Tseng CTK, Tseng J, Perrone L, Worthy M, Popov V, Peters CJ. Apical entry and release of severe acute respiratory syndrome-associated coronavirus in polarized Calu-3 lung epithelial cells. J Virol. 2005;79:9470–9479
  39. Khodarev NN, Yu J, Labay E, Darga T, Brown CK, Mauceri HJ, et al. Tumour-endothelium interactions in co-culture: Coordinated changes of gene expression profiles and phenotypic properties of endothelial cells. J Cell Sci. 2003;116:1013–1022
  40. Kitchens KM, El Sayed MEH, Ghandehari H. Transepithelial and endothelial transport of poly (amidoamine) dendrimers. Adv Drug Deliv Rev. 2005;57:2163–2176
  41. Bur M, Huwer H, Lehr CM, Hagen N, Guldbrandt M, Kim KJ, et al. Assessment of transport rates of proteins and peptides across primary human alveolar epithelial cell monolayers. Eur J Pharm Sci. 2006;28:196–203
  42. Liu DZ, Lecluyse EL, Thakker DR. Dodecylphosphocholine-mediated enhancement of paracellular permeability and cytotoxicity in Caco-2 cell monolayers. J Pharm Sci. 1999;88:1161–1168
  43. Woclawek-Potocka I, Korzekwa A, Bah MM, Skarzynski DJ, Acosta TJ, Shibaya M, et al. Phytoestrogens modulate prostaglandin production in bovine endometrium: Cell type specificity and intracellular mechanisms. Exp Biol Med (Maywood). 2005;230:326–333
  44. Agerholm JS, Jensen NE, Dantzer V, Jensen HE, Aarestrup FM. Experimental infection of pregnant cows with Bacillus licheniformis bacteria. Vet Pathol. 1999;36:191–201
  45. Dubey JP, Buxton D, Wouda W. Pathogenesis of bovine neosporosis. J Comp Pathol. 2006;134:267–289

PII: S0143-4004(07)00184-1

doi: 10.1016/j.placenta.2007.07.002

Placenta
Volume 28, Issue 11 , Pages 1110-1117 , November 2007

Article Tools

* Image Usage & Resolution