Placenta
Volume 29, Issue 6 , Pages 531-538 , June 2008

Cultured Bovine Trophoblast Cells Differentially Express Genes Encoding Key Steroid Synthesis Enzymes

,Accepted 21 March 2008.

References 

  1. Klisch K, Pfarrer C, Schuler G, Hoffmann B, Leiser R. Tripolar acytokinetic mitosis and formation of feto–maternal syncytia in the bovine placentome: different modes of the generation of multinuclear cells. Anat Embryol (Berl). 1999;200:229–237
  2. Hashizume K, Ushizawa K, Patel OV, Kizaki K, Imai K, Yamada O, et al. Gene expression and maintenance of pregnancy in bovine: roles of trophoblastic binucleate cell-specific molecules. Reprod Fertil Dev. 2007;19:79–90
  3. Hoffmann B, Schuler G. The bovine placenta; a source and target of steroid hormones: observations during the second half of gestation. Domest Anim Endocrinol. 2002;23:309–320
  4. Schuler G, Ozalp GR, Hoffmann B, Harada N, Browne P, Conley AJ. Reciprocal expression of 17alpha-hydroxylase-C17,20-lyase and aromatase cytochrome P450 during bovine trophoblast differentiation: a two-cell system drives placental oestrogen synthesis. Reproduction. 2006;131:669–679
  5. Schuler G, Wirth C, Teichmann U, Failing K, Leiser R, Thole H, et al. Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition. Biol Reprod. 2002;66:976–982
  6. Fürbass R, Said HM, Schwerin M, Vanselow J. Chromatin structure of the bovine Cyp19 promoter 1.1: DNaseI hypersensitive sites and DNA hypomethylation correlate with placental expression. Eur J Biochem. 2001;268:1222–1227
  7. Fürbass R, Selimyan R, Vanselow J. DNA methylation and chromatin accessibility of the proximal Cyp19 promoter region 1.5/2 correlate with expression levels in sheep placentomes. Mol Reprod Dev. 2008;75:1–7
  8. Munson L, Chandler SK, Schlafer DH. Long term culture of bovine trophoblast cells. J Tissue Cult Methods. 1988;11:123–128
  9. Bridger PS, Haupt S, Klisch K, Leiser R, Tinneberg HR, Pfarrer C. Validation of primary epitheloid cell cultures isolated from bovine placental caruncles and cotyledons. Theriogenology. 2007;68:592–603
  10. Nakano H, Takahashi T, Imai K, Hashizume K. Expression of placental lactogen and cytokeratin in bovine placental binucleate cells in culture. Cell Tissue Res. 2001;303:263–270
  11. Landim LP, Miglino MA, Pfarrer C, Ambrosio CE, Garcia JM. Culture of mature trophoblastic giant cells from bovine placentomes. Anim Reprod Sci. 2007;98:357–364
  12. Zeiler M, Leiser R, Johnson GA, Tinneberg HR, Pfarrer C. Development of an in vitro model for bovine placentation: a comparison of the in vivo and in vitro expression of integrins and components of extracellular matrix in bovine placental cells. Cells Tissues Organs. 2007;186:229–242
  13. Shimada A, Nakano H, Takahashi T, Imai K, Hashizume K. Isolation and characterization of a bovine blastocyst-derived trophoblastic cell line, BT-1: development of a culture system in the absence of feeder cell. Placenta. 2001;22:652–662
  14. Klisch K, Leiser R. In bovine binucleate trophoblast giant cells, pregnancy-associated glycoproteins and placental prolactin-related protein-I are conjugated to asparagine-linked N-acetylgalactosaminyl glycans. Histochem Cell Biol. 2003;119:211–217
  15. Kadyrov M, Garnier Y, Gantert M, Kramer B, Kaufmann P, Huppertz B. Cytokeratin antibodies as differential markers of trophoblast and fetomaternal syncytial plaques in the sheep placentome. Placenta. 2007;28:1107–1109
  16. Nakano H, Shimada A, Imai K, Takahashi T, Hashizume K. Association of Dolichos biflorus lectin binding with full differentiation of bovine trophoblast cells. Reproduction. 2002;124:581–592
  17. Nakano H, Shimada A, Imai K, Takezawa T, Takahashi T, Hashizume K. Bovine trophoblastic cell differentiation on collagen substrata: formation of binucleate cells expressing placental lactogen. Cell Tissue Res. 2002;307:225–235

PII: S0143-4004(08)00112-4

doi: 10.1016/j.placenta.2008.03.004

Placenta
Volume 29, Issue 6 , Pages 531-538 , June 2008

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