Placenta
Volume 30, Issue 1 , Pages 11-14, January 2009

Over-expression of SOCS-3 Gene Promotes IL-10 Production by JEG-3 Trophoblast Cells☆

  • Q. Dong

      Affiliations

    • Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
    • Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China
    • ,
  • R. Fan

      Affiliations

    • Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
    • ,
  • S. Zhao

      Affiliations

    • Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
    • ,
  • Y. Wang

      Affiliations

    • Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
    • Corresponding Author InformationCorresponding author. Department of Obstetrics and Gynecology, LSUHSC-Shreveport, PO Box 33932, 1501 Kings Highway, Shreveport, LA 71130, USA. Tel.: +1 318 675 5379; fax: +1 318 675 4671.

Accepted 6 October 2008. published online 27 November 2008.

Abstract 

Suppressor of cytokine signaling-3 (SOCS-3) plays an important role in negative regulation of inflammatory response. Evidence has shown that SOCS-3 and IL-10 expressions were significantly reduced in placental trophoblasts from preeclampsia. IL-10 is an anti-inflammatory cytokine. In this study, we sought to determine if enhance SOCS-3 expression could affect IL-10 production in placental trophoblasts. Placental JEG-3 cells were used. Over-expression of SOCS-3 was generated by transfection of JEG-3 cells with a green fluorescent protein (GFP) tagged SOCS-3 gene, SOCS-3/ZsGreen1, by siPORT lipid transfection. Cells transfected with ZsGreen1 vector only was used as control. Our results showed that IL-6 production was reduced in cells over-expressed with SOCS-3. Moreover, SOCS-3 transfected cells produced more IL-10 when stimulated with IL-6. The increased IL-10 production by JEG-3 cells was in a dose-dependent manner, p<0.05. Our data suggested that enhanced SOCS-3 gene expression could promote IL-10 production by placental trophoblast cells, suggesting that SOCS-3 may play an important role in regulation of cytokine induced anti-inflammatory response in placental trophoblasts.

Keywords: SOCS-3, IL-6, IL-10, Trophoblasts

To access this article, please choose from the options below

Login to an existing account or Register a new account.

  • Purchase this article for 31.50 USD (You must login/register to purchase this article)

    Online access for 24 hours. The PDF version can be downloaded as your permanent record.

  • Subscribe to this title

    Get unlimited online access to this article and all other articles in this title 24/7 for one year.

  • Claim access now

    For current subscribers with Society Membership or Account Number.

  • Visit SciVerse ScienceDirect to see if you have access via your institution.
 

☆ This study was supported in part by grants from National Institute of Health, NICHD (HD36822) and NHLBI (HL65997), and presented at the 55th Annual Meeting for Society for Gynecologic Investigation, March 26–29, 2008, San Diego, CA.

PII: S0143-4004(08)00336-6

doi:10.1016/j.placenta.2008.10.008

Placenta
Volume 30, Issue 1 , Pages 11-14, January 2009

Article Tools

* Image Usage & Resolution