Placenta
Volume 30, Issue 2 , Pages 124-129 , February 2009

Methods for siRNA-mediated Reduction of mRNA and Protein Expression in Human Placental Explants, Isolated Primary Cells and Cell Lines

,Accepted 7 October 2008.

References 

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  2. Hannon GJ. RNA interference. Nature. 2002;418:244
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  4. Gilmore IR, Fox SP, Hollins AJ, Sohail M, Akhtar S. The design and exogenous delivery of siRNA for post-transcriptional gene silencing. Journal of Drug Targeting. 2004;12:315
  5. Leitner K, Szlauer R, Ellinger I, Ellinger A, Zimmer KP, Fuchs R. Placental alkaline phosphatase expression at the apical and basal plasma membrane in term villous trophoblasts. The Journal of Histochemistry and Cytochemistry. 2001;49:1155
  6. Lacey HA, Nolan T, Greenwood SL, Glazier JD, Sibley CP. Gestational profile of Na+/H+ exchanger and Cl−/HCO3− anion exchanger mRNA expression in placenta using real-time QPCR. Placenta. 2005;26:93
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  9. Greenwood SL, Brown PD, Edwards D, Sibley CP. Patch clamp studies of human placental cytotrophoblast cells in culture. Placenta. 1993;14:53
  10. Forbes K, Westwood M, Baker PN, Aplin JD. Insulin-like growth factor I and II regulate the life cycle of trophoblast in the developing human placenta. American Journal of Physiology Cell Physiology. 2008;294:C1313
  11. Choy MY, St Whitley G, Manyonda IT. Efficient, rapid and reliable establishment of human trophoblast cell lines using poly-L-ornithine. Early Pregnancy. 2000;4:124
  12. Shyu MK, Lin MC, Shih JC, Lee CN, Huang J, Liao CH, et al. Mucin 15 is expressed in human placenta and suppresses invasion of trophoblast-like cells in vitro. Human Reproduction. 2007;22:2723
  13. Ito T, Nomura S, Okada M, Katsumata Y, Iwase A, Kikkawa F, et al. Transcriptional regulation of human placental leucine aminopeptidase/oxytocinase gene. Molecular Human Reproduction. 2001;7:887
  14. Rolfo A, Post M, Caniggia I. Evidence for direct HIF regulation of PHD2 and PHD3 in human JEG-3 choriocarcinoma cells. Placenta. 2007;28:A648
  15. Heazell A, Baczyck D, Dunk C, Perkins J, Jones C, Baker P, et al. An imbalance between p53 and Mdm2 induces apoptosis in trophoblast. Placenta. 2007;28:A804
  16. Zhang M, Guller S, Huang Y. Method to enhance transfection efficiency of cell lines and placental fibroblasts. Placenta. 2007;28:779
  17. Whither RNAi?. Nature Cell Biology. 2003;5:489

PII: S0143-4004(08)00337-8

doi: 10.1016/j.placenta.2008.10.003

Placenta
Volume 30, Issue 2 , Pages 124-129 , February 2009

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