AMP-Deaminase from Developing Human Placenta

AMP-deaminase from developing human placenta. a) Western blot analysis of homogenates. 15 μg of placental homogenate protein was applied onto the gel during SDS-PAG electrophoresis. Rabbit antibodies against AMP-deaminase purified from human term (∼40 week-old) placenta were used for immunological staining of the blot. Lane 1 – colour protein standards-Protein Ladder, 11–170 kDa, lane 2 – homogenate from 25 week-old placenta, lane 3 – homogenate from 33 week-old placenta, lane 4 - homogenate from term placenta (∼40 week-old); b) Densitometric analysis of Western blot results. The intensity of immunological was evaluated by using of Quantity One (Biorad) program. Statistical significance of differences between values: (**) p < 0.001, (*) p < 0.005.