Placenta
Volume 31, Issue 9 , Pages 803-810 , September 2010

Effect of glucocorticoids on regulation of placental multidrug resistance phosphoglycoprotein (P-gp) in the mouse

  • S. Petropoulos

      Affiliations

    • Department of Physiology, Faculty of Medicine, University of Toronto, Medical Sciences Building, 1 King’s College Circle, Toronto, Ontario M5S 1A8, Canada
    • ,
  • W. Gibb

      Affiliations

    • Department of Obstetrics and Gynecology, University of Ottawa, Canada
    • Department of Cellular and Molecular Medicine, University of Ottawa, Canada
    • ,
  • S.G. Matthews

      Affiliations

    • Department of Physiology, Faculty of Medicine, University of Toronto, Medical Sciences Building, 1 King’s College Circle, Toronto, Ontario M5S 1A8, Canada
    • Department of Obstetrics and Gynecology, University of Toronto, Medical Sciences Building, 1 King’s College Circle, Toronto, Ontario M5S 1A8, Canada
    • Department of Medicine, University of Toronto, Medical Sciences Building, 1 King’s College Circle, Toronto, Ontario M5S 1A8, Canada
    • Corresponding Author InformationCorresponding author. Department of Physiology, Faculty of Medicine, University of Toronto, Medical Sciences Building, 1 King’s College Circle, Toronto, Ontario M5S 1A8, Canada. Tel.: +1 416 978 1974; fax: +1 416 978 4940.

,Accepted 22 June 2010.

  • Image Result

    Tissue weight following daily injections of dexamethasone (0.1 mg/kg, 1 mg/kg) or vehicle (VEH) from embryonic day (E) 9.5–E15.5 or E12.5–E18.5. (A) Placenta weight following dexamethasone treatment E

    Tissue weight following daily injections of dexamethasone (0.1 mg/kg, 1 mg/kg) or vehicle (VEH) from embryonic day (E) 9.5–E15.5 or E12.5–E18.5. (A) Placenta weight following dexamethasone treatment E15.5 (vehicle (VEH) n = 10 litters/group; 0.1 mg/kg, n = 6 litters/group; 1 mg/kg, n = 5 litters/group) and (B) E18.5 (VEH, n = 12 litters/group; 0.1 mg/kg, n = 6 litters/group; 1 mg/kg, n = 5 litters/group). (C) Fetal body weight following dexamethasone treatment on E15.5 (VEH, n = 10 litters/group; 0.1 mg/kg, n = 7 litters/group; 1 mg/kg, n = 5 litters/group) and (D) E18.5 (VEH, n = 9 litters/group; 0.1 mg/kg, n = 5 litters/group; 1 mg/kg, n = 5 litters/group). Bar represents mean ± SEM tissue weight in grams (g). *P < 0.05, ***P < 0.001.

  • Image Result
    Placental Abcb1 mRNA expression following dexamethasone 0.1 mg/kg (DEX 0.1), dexamethasone 1 mg/kg (DEX 1) or vehicle (VEH 0.1 or VEH 1). (A) Abcb1a mRNA on embryonic day (E)15.5 (DEX 0.1, n = 10 plac

    Placental Abcb1 mRNA expression following dexamethasone 0.1 mg/kg (DEX 0.1), dexamethasone 1 mg/kg (DEX 1) or vehicle (VEH 0.1 or VEH 1). (A) Abcb1a mRNA on embryonic day (E)15.5 (DEX 0.1, n = 10 placentae/group; DEX 1, n = 5 placentae/group and their corresponding vehicle (VEH) 0.1, n = 11 placentae/group; VEH 1, n = 8 placentae/group), (B) Abcb1b mRNA on E15.5 (DEX 0.1, n = 10 placentae/group; DEX 1, n = 10 placentae/group and their corresponding vehicle VEH 0.1, n = 13 placentae/group; VEH 1, n = 9 placentae/group), (C) Abc1b1a mRNA on E18.5 (DEX 0.1, n = 4 placentae/group; DEX 1, n = 9 placentae/group and their corresponding vehicle VEH 0.1, n = 8 placentae/group; VEH 1, n = 10 placentae/group) and (D) Abcb1b mRNA on E18.5 (DEX 0.1, n = 5 placentae/group; DEX 1, n = 5 placentae/group and their corresponding vehicle VEH 0.1, n = 8 placentae/group; VEH 1, n = 7 placentae/group). Bar represents mean ± SEM expressed as relative units of mRNA standardized against Gapdh. Treatment data was then normalized to corresponding vehicle (run in parallel to treatment). *P < 0.05.

  • Image Result
    Placental P-gp on embryonic day (E) 15.5 from (A) dexamethasone 0.1 mg/kg (DEX 0.1) or vehicle (VEH; n = 5 placentae/group) or (B) dexamethasone 1 mg/kg (DEX 1) or vehicle (VEH; n = 5 placentae/group)

    Placental P-gp on embryonic day (E) 15.5 from (A) dexamethasone 0.1 mg/kg (DEX 0.1) or vehicle (VEH; n = 5 placentae/group) or (B) dexamethasone 1 mg/kg (DEX 1) or vehicle (VEH; n = 5 placentae/group) treated dams. (C) Representative blot of placental P-gp on E18.5 following vehicle (VEH) and dexamethasone (DEX) 1 mg/kg treatment. Placental P-gp on E18.5 from (D) dexamethasone 0.1 mg/kg (DEX 0.1) or vehicle (VEH; n = 5 placentae/group) or (E) dexamethasone 1 mg/kg (DEX 1) or vehicle (VEH; n = 5 placentae/group) treated dams. Bar represents mean ± SEM expressed as relative optical density (ROD) standardized against β-actin. *P < 0.05, **P < 0.01.

  • Image Result
    Transplacental transfer of [3H]digoxin (50 μg/kg) to the ‘fetal-unit’ on: (A) embryonic day (E)15.5 after daily injections of dexamethasone (DEX) 0.1 mg/kg (n = 7 litters/group), 1 mg/kg (n = 5 litter

    Transplacental transfer of [3H]digoxin (50 μg/kg) to the ‘fetal-unit’ on: (A) embryonic day (E)15.5 after daily injections of dexamethasone (DEX) 0.1 mg/kg (n = 7 litters/group), 1 mg/kg (n = 5 litters/group) or vehicle (VEH; n = 5 litters/group) from E9.5-E15.5. (B) E18.5 after daily injections with dexamethasone (0.1 mg/kg (n = 6 litters/group), 1 mg/kg (n = 7 litters/group)) or vehicle (n = 10 litters/group) from E12.5-E18.5. Values are expressed as treatment group means. Bar represents mean ± SEM drug ratio (DPM/g fetal tissue, membranes and amniotic fluid: DPM/ml maternal plasma). ***P < 0.001.

PII: S0143-4004(10)00244-4

doi: 10.1016/j.placenta.2010.06.014

Placenta
Volume 31, Issue 9 , Pages 803-810 , September 2010

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