Placenta
Volume 31, Issue 10 , Pages 928-936 , October 2010

Differential expression of functional nucleoside transporters in non-differentiated and differentiated human endothelial progenitor cells

  • E. Guzmán-Gutiérrez

      Affiliations

    • Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, Universidad de Concepción, Chile
    • Actual address: Cellular and Molecular Physiology Laboratory (CMPL) & Perinatology Research Laboratory (PRL), Division of Obstetrics and Gynaecology, Medical Research Centre (CIM), School of Medicine, Faculty of Medicine, Pontificia Universidad Católica de Chile, P.O. Box 114-D, Santiago, Chile.
    • ,
  • C. Sandoval

      Affiliations

    • Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, Universidad de Concepción, Chile
    • ,
  • E. Nova

      Affiliations

    • Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, Universidad de Concepción, Chile
    • ,
  • J.L. Castillo

      Affiliations

    • Department of Medical Specialties, Faculty of Medicine, Universidad de Concepción, Chile
    • ,
  • J.C. Vera

      Affiliations

    • Department of Physiopathology, Faculty of Biological Sciences, Universidad de Concepción, Chile
    • ,
  • L. Lamperti

      Affiliations

    • Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, Universidad de Concepción, Chile
    • ,
  • B. Krause

      Affiliations

    • Cellular and Molecular Physiology Laboratory (CMPL) & Perinatology Research Laboratory (PRL), Division of Obstetrics and Gynaecology, Medical Research Centre (CIM), School of Medicine, Faculty of Medicine, Pontificia Universidad Católica de Chile, P.O. Box 114-D, Santiago, Chile
    • ,
  • C. Salomón

      Affiliations

    • Cellular and Molecular Physiology Laboratory (CMPL) & Perinatology Research Laboratory (PRL), Division of Obstetrics and Gynaecology, Medical Research Centre (CIM), School of Medicine, Faculty of Medicine, Pontificia Universidad Católica de Chile, P.O. Box 114-D, Santiago, Chile
    • ,
  • C. Sepúlveda

      Affiliations

    • Cellular and Molecular Physiology Laboratory (CMPL) & Perinatology Research Laboratory (PRL), Division of Obstetrics and Gynaecology, Medical Research Centre (CIM), School of Medicine, Faculty of Medicine, Pontificia Universidad Católica de Chile, P.O. Box 114-D, Santiago, Chile
    • ,
  • C. Aguayo

      Affiliations

    • Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, Universidad de Concepción, Chile
    • These authors contributed equally to this study.
    • ,
  • L. Sobrevia

      Affiliations

    • Cellular and Molecular Physiology Laboratory (CMPL) & Perinatology Research Laboratory (PRL), Division of Obstetrics and Gynaecology, Medical Research Centre (CIM), School of Medicine, Faculty of Medicine, Pontificia Universidad Católica de Chile, P.O. Box 114-D, Santiago, Chile
    • Corresponding Author InformationCorresponding author. Tel.: +56 2 3548118; fax: +56 2 6321924.
    • These authors contributed equally to this study.

,Accepted 29 July 2010.

References 

  1. Tepper OM, Capla JM, Galiano RD, Ceradini DJ, Callaghan MJ, Kleinman ME, et al. Adult vasculogenesis occurs through in situ recruitment, proliferation, and tubulization of circulating bone marrow-derived cells. Blood. 2005;105:1068–1077
  2. Sipos PI, Crocker IP, Hubel CA, Baker PN. Endothelial progenitor cells: their potential in the placental vasculature and related complications. Placenta. 2010;31:1–10
  3. Matsubara K, Abe E, Matsubara Y, Kameda K, Ito M. Circulating endothelial progenitor cells during normal pregnancy and pre-eclampsia. Am J Reprod Immunol. 2006;56:79–85
  4. Riksen NP, Rongen GA, Yellon D, Smits P. Human in vivo research on the vascular effects of adenosine. Eur J Pharmacol. 2008;585:220–227
  5. Rose JB, Coe IR. Physiology of nucleoside transporters: back to the future. Physiology. 2008;23:41–48
  6. Westermeier F, Puebla C, Vega JL, Farias M, Escudero C, Casanello P, et al. Equilibrative nucleoside transporters in fetal endothelial dysfunction in diabetes mellitus and hyperglycaemia. Curr Vasc Pharmacol. 2009;7:435–439
  7. Casanello P, Escudero C, Sobrevia L. Equilibrative nucleoside (ENTs) and cationic amino acid (CATs) transporters: implications in foetal endothelial dysfunction in human pregnancy diseases. Curr Vasc Pharmacol. 2007;5:69–84
  8. Escudero C, Puebla C, Westermeier F, Sobrevia L. Potential cell signalling mechanisms involved in differential placental angiogenesis in mild and severe pre-eclampsia. Curr Vasc Pharmacol. 2009;7:475–485
  9. Pearson JD. Endothelial progenitor cells – hype or hope?. J Thromb Haemost. 2009;7:255–262
  10. Baldwin SA, Beal PR, Yao SY, King AE, Cass CE, Young JD. The equilibrative nucleoside transporter family, SLC29. Pflugers Arch. 2004;447:735–743
  11. Molina-Arcas M, Casado FJ, Pastor-Anglada M. Nucleoside transporter proteins. Curr Vasc Pharmacol. 2009;7:426–434
  12. Kermani P, Leclerc G, Martel R, Fareh J. Effect of ionizing radiation on thymidine uptake, differentiation, and VEGFR2 receptor expression in endothelial cells: the role of VEGF(165). Int J Radiat Oncol Biol Phys. 2001;50:213–220
  13. Shord SS, Camp JR, Young LA. Paclitaxel decreases the accumulation of gemcitabine and its metabolites in human leukemia cells and primary cell cultures. Anticancer Res. 2005;25:4165–4171
  14. Shaked Y, Henke E, Roodhart JM, Mancuso P, Langenberg MH, Colleoni M, et al. Rapid chemotherapy-induced acute endothelial progenitor cell mobilization: implications for antiangiogenic drugs as chemosensitizing agents. Cancer Cell. 2008;14:263–273
  15. Montesinos MC, Shaw JP, Yee H, Shamamian P, Cronstein BN. Adenosine A(2A) receptor activation promotes wound neovascularization by stimulating angiogenesis and vasculogenesis. Am J Pathol. 2004;164:1887–1892
  16. Ryzhov S, Solenkova NV, Goldstein AE, Lamparter M, Fleenor T, Young PP, et al. Adenosine receptor-mediated adhesion of endothelial progenitors to cardiac microvascular endothelial cells. Circ Res. 2008;102:356–363
  17. Katebi M, Soleimani M, Cronstein BN. Adenosine A2A receptors play an active role in mouse bone marrow-derived mesenchymal stem cell development. J Leukoc Biol. 2009;85:438–444
  18. Asahara T, Murohara T, Sullivan A, Silver M, van der Zee R, Li T, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science. 1997;275:964–967
  19. Lin Y, Weisdorf DJ, Solovey A, Hebbel RP. Origins of circulating endothelial cells and endothelial outgrowth from blood. J Clin Invest. 2000;105:71–77
  20. Bellik L, Ledda F, Parenti A. Morphological and phenotypical characterization of human endothelial progenitor cells in an early stage of differentiation. FEBS Lett. 2005;579:2731–2736
  21. Farías M, Puebla C, Westermeier F, Jo MJ, Pastor-Anglada M, Casanello P, et al. Nitric oxide reduces SLC29A1 promoter activity and adenosine transport involving transcription factor complex hCHOP-C/EBPα in human umbilical vein endothelial cells from gestational diabetes. Cardiovasc Res. 2010;86:45–54
  22. Copeland RA. Determination of serum protein binding affinity of inhibitors from analysis of concentration-response plots in biochemical activity assays. J Pharm Sci. 2000;89:1000–1007
  23. Eltzschig HK, Abdulla P, Hoffman E, Hamilton KE, Daniels D, Schonfeld C, et al. HIF-1-dependent repression of equilibrative nucleoside transporter (ENT) in hypoxia. J Exp Med. 2005;202:1493–1505
  24. King AE, Ackley MA, Cass CE, Young JD, Baldwin SA. Nucleoside transporters: from scavengers to novel therapeutic targets. Trends Pharmacol Sci. 2006;27:416–425
  25. Sen RP, Sobrevia L, Delicado EG, Yudilevich D, Miras-Portugal MT. Bovine adrenal endothelial cells express nucleoside transporters nonregulated by protein kinases A and C. Am J Physiol. 1996;271:C504–C510
  26. Beck DW, Vinters HV, Moore SA, Hart MN, Cancilla PA. Uptake of adenosine by cultured cerebral vascular smooth muscle cells. J Neurochem. 1983;41:939–941
  27. Abd-Elfattah AS, Jessen ME, Lekven J, Wechsler AS. Differential cardioprotection with selective inhibitors of adenosine metabolism and transport: role of purine release in ischemic and reperfusion injury. Mol Cell Biochem. 1998;180:179–191
  28. Aguayo C, Sobrevia L. Nitric oxide, cGMP and cAMP modulate nitrobenzylthioinosine-sensitive adenosine transport in human umbilical artery smooth muscle cells from subjects with gestational diabetes. Exp Physiol. 2000;85:399–409
  29. Bone DB, Hammond JR. Nucleoside and nucleobase transporters of primary human cardiac microvascular endothelial cells: characterization of a novel nucleobase transporter. Am J Physiol Heart Circ Physiol. 2007;293:H3325–H3332
  30. Archer RG, Pitelka V, Hammond JR. Nucleoside transporter subtype expression and function in rat skeletal muscle microvascular endothelial cells. Br J Pharmacol. 2004;143:202–214
  31. Chishty M, Begley DJ, Abbott NJ, Reichel A. Functional characterisation of nucleoside transport in rat brain endothelial cells. Neuroreport. 2003;14:1087–1090
  32. Redzic ZB, Biringer J, Barnes K, Baldwin SA, Al-Sarraf H, Nicola PA, et al. Polarized distribution of nucleoside transporters in rat brain endothelial and choroid plexus epithelial cells. J Neurochem. 2005;94:1420–1426
  33. Barnes K, Dobrzynski H, Foppolo S, Beal PR, Ismat F, Scullion ER, et al. Distribution and functional characterization of equilibrative nucleoside transporter-4, a novel cardiac adenosine transporter activated at acidic pH. Circ Res. 2006;99:510–519
  34. Fernández P, Guzmán-Gutiérrez E, Caviedes L, Aguilera V, Lamperti L, Aguayo C. Activation of adenosine receptor induces mobilization of human endothelial progenitor cells (Abstract). Placenta, in press.
  35. Podgorska M, Kocbuch K, Grden M, Szutowicz A, Pawelczyk T. Reduced ability to release adenosine by diabetic rat cardiac fibroblasts due to altered expression of nucleoside transporters. J Physiol. 2006;576:179–189
  36. Flanagan SA, Meckling KA. Nucleoside transporter expression and activity is regulated during granulocytic differentiation of NB4 cells in response to all-trans-retinoic acid. Leuk Res. 2007;31:955–968
  37. Jarvis SM, Young JD. Nucleoside translocation in sheep reticulocytes and fetal erythrocytes: a proposed model for the nucleoside transporter. J Physiol. 1982;324:47–66
  38. Lee CW. Decrease in equilibrative uridine transport during monocytic differentiation of HL-60 leukaemia: involvement of protein kinase C. Biochem J. 1994;300:407–412
  39. Myatt L, Webster RP. Vascular biology of preeclampsia. J Thromb Haemost. 2009;7:375–384

PII: S0143-4004(10)00285-7

doi: 10.1016/j.placenta.2010.07.016

Placenta
Volume 31, Issue 10 , Pages 928-936 , October 2010

Article Tools

* Image Usage & Resolution