Short communicationComparative phenotypic characterization of human cord blood monocytes and placental macrophages at term
Introduction
There is now little doubt that placental macrophages (Hofbauer cells) are of fetal, rather than maternal, origin. At the early gestational stage, macrophages differentiate from mesenchymal cells within the stroma of developing chorionic villi [1], [2], [3]. Later, the arrangement of embryo placental blood circulation allows the replenishment of the tissue macrophage population by means of extra-placental hematopoietic sources. The existence of transitional forms between monocytes and macrophages suggests that bone marrow-originated monocytes are predecessors of placental macrophages [4]. In accordance with the current concept, the macrophage pool in placenta villous tissue is sustained to a great extent by recruiting fetal blood monocytes [5], [6], [7]. However, the phenotypic and functional properties of mononuclear phagocytes in the placenta remain poorly characterized.
In this study we evaluated the expression of surface molecules in cord blood monocytes and placental macrophages in order to further elucidate their phenotypes and to reveal differences in the antigenic profiles between these related cell populations.
Section snippets
Methods
Placental tissue and cord blood were obtained after elective caesarian section at term for uncomplicated pregnancy (37–41 weeks). All sample collections and experiments were approved by the Ethic Committee of the Institute for Obstetrics and Gynecology. Clinical characteristics of the placentas studied are given in Table 1 [8].
Cord blood was diluted with Cell Wash buffer (BD Biosciences, San Diego, CA) and subjected to density gradient centrifugation on Histopaque®-1077 (Sigma, St Louis, MO)
Results and discussion
The results of the study are shown in Table 2.
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